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aavrg hsyn mcherry  (Addgene inc)


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    Addgene inc aavrg hsyn mcherry
    Aavrg Hsyn Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ubch5a/pm40734274-350-21-23?v=Addgene+inc
    Average 93 stars, based on 3 article reviews
    aavrg hsyn mcherry - by Bioz Stars, 2026-07
    93/100 stars

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    a Schematic illustration of the catalytic mechanisms employed by major E3 ligase families: RING, HECT, and RBR. Acceptor lysine (on E3 or on substrate) is shown in orange. b Schematic showing the experimental workflow of the E2 discharge assay using free amino acids. c E2 discharge assay using UbcH8∼ISG15 in the presence of various amino acids (50 mM) d E2 discharge assay using UbcH8∼ISG15 in the presence of increasing concentrations of L-lys as indicated. e E2 discharge assay using UbcH7∼Ub in the presence of various amino acids (50 mM f E2 discharge assay using <t>UbcH5A∼Ub</t> in the presence of various amino acids (50 mM)
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    a Schematic illustration of the catalytic mechanisms employed by major E3 ligase families: RING, HECT, and RBR. Acceptor lysine (on E3 or on substrate) is shown in orange. b Schematic showing the experimental workflow of the E2 discharge assay using free amino acids. c E2 discharge assay using UbcH8∼ISG15 in the presence of various amino acids (50 mM) d E2 discharge assay using UbcH8∼ISG15 in the presence of increasing concentrations of L-lys as indicated. e E2 discharge assay using UbcH7∼Ub in the presence of various amino acids (50 mM f E2 discharge assay using <t>UbcH5A∼Ub</t> in the presence of various amino acids (50 mM)
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    a Schematic illustration of the catalytic mechanisms employed by major E3 ligase families: RING, HECT, and RBR. Acceptor lysine (on E3 or on substrate) is shown in orange. b Schematic showing the experimental workflow of the E2 discharge assay using free amino acids. c E2 discharge assay using UbcH8∼ISG15 in the presence of various amino acids (50 mM) d E2 discharge assay using UbcH8∼ISG15 in the presence of increasing concentrations of L-lys as indicated. e E2 discharge assay using UbcH7∼Ub in the presence of various amino acids (50 mM f E2 discharge assay using UbcH5A∼Ub in the presence of various amino acids (50 mM)

    Journal: bioRxiv

    Article Title: ISGylation Mechanism Uncovers Conformational Specificity for HECT-family E3 ligase

    doi: 10.1101/2025.09.26.678755

    Figure Lengend Snippet: a Schematic illustration of the catalytic mechanisms employed by major E3 ligase families: RING, HECT, and RBR. Acceptor lysine (on E3 or on substrate) is shown in orange. b Schematic showing the experimental workflow of the E2 discharge assay using free amino acids. c E2 discharge assay using UbcH8∼ISG15 in the presence of various amino acids (50 mM) d E2 discharge assay using UbcH8∼ISG15 in the presence of increasing concentrations of L-lys as indicated. e E2 discharge assay using UbcH7∼Ub in the presence of various amino acids (50 mM f E2 discharge assay using UbcH5A∼Ub in the presence of various amino acids (50 mM)

    Article Snippet: The following plasmids were obtained from Addgene: UBE1 (Addgene plasmid #34965; gift from Cynthia Wolberger), UbcH5A (Addgene plasmid #15782; gift from Wade Harper), and NEDD4L (Addgene plasmid #165103; gift from Arthur Haas).

    Techniques: